A stable synthetic retinoid is designed to potently induce and reliably control stem cell differentiation.
Controlling cell differentiation in a predictable way is a major challenge in stem cell research. Although natural retinoids such as all-trans retinoic acid (ATRA) can be used to trigger neuronal stem cell differentiation, they are inherently unstable, leading to partially differentiated cultures and variable results. This reagent, which remains chemically and physically stable under normal laboratory conditions, overcomes these challenges and offers additional useful features for stem cell differentiation.
Manufacturer says the reagent has been proven in independent testing** to potently induce and reliably control stem cell differentiation.
** References: 1. Retinoid supplementation of differentiating human neural progenitors and embryonic stem cells leads to enhanced neurogenesis in vitro. Christie VB, Maltman DJ, Henderson AP, Whiting A, Marder TB, Lako M, Przyborski SA. (J Neurosci Methods. 2010 Nov 30;193(2):239-45). 2. Synthetic retinoids: structure-activity relationships. Barnard JH, Collings JC, Whiting A, Przyborski SA, Marder TB. (Chemistry. 2009 Nov 2;15(43):11430-42). 3. Proteomic profiling of the stem cell response to retinoic acid and synthetic retinoid analogues: identification of major retinoid-inducible proteins. Maltman DJ, Christie VB, Collings JC, Barnard JH, Fenyk S, Marder TB, Whiting A, Przyborski SA. Mol Biosyst. 2009 May;5(5):458-71.