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Listeria assay earns AOAC PTM approval


The Molecular Detection Assay Listeria monocytogenes test kit helps food processors and other parties quickly and accurately detect this pathogen. The kit has now received approval by the AOAC Performance Tested Methods program (Certification #051401). The approval means it is now considered equivalent or better than standard reference methods for select food matrices by the international organization. The assay provides rapid results in as little as 24 hours of enrichment – days faster than the standard methods.

Food samples analyzed during the validation study include full-fat cottage cheese, chocolate milk, beef hot dogs, deli turkey, cold smoked salmon, bagged raw spinach, romaine lettuce and whole cantaloupe. Testing also covered environmental surfaces including stainless steel and concrete.

In a related development, the Molecular Detection Assay Listeria species has received a matrix extension from the same program (Certification #081203). The test kit has received the PTM extension for the following matrices: full fat cottage cheese, beef hot dogs, deli turkey, cold cooked salmon, bagged raw spinach, whole cantaloupe, as well as environmental surfaces including stainless steel and concrete.

The manufacturer says achieving AOAC PTM status for these assays required a rigorous, independent laboratory examination of its molecular test method’s ability to accurately detect Listeria monocytogenes and Listeria spp. within a variety of intentionally contaminated food matrices. No statistically significant differences were found in the sample results between the 3M Molecular Detection Assays when compared to conventional reference methods.

The 3M Molecular Detection System is based on isothermal DNA amplification and bioluminescence detection technologies. It is designed around food processors’ needs for a real-time pathogen detection approach that’s faster and simpler while also more accurate.


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