A comprehensive Fluorescence Method Development Handbook has been released by
Thermo Fisher Scientific. Fluorescence method development is much more complex compared to other optical detectors such as UV or refractive index detection. For optimum results, it is important to optimize the different parameters in a systematic way.
This 16-page guide explains fluorescence theory and its effects on experimental results during method development. It also demonstrates how to benefit from the unique features of the company’s fluorescence detectors like the Thermo Scientific Dionex FLD-3000 Series. The handbook is intended to be a source of information for users of their HPLC systems and other chromatographers generally interested in fluorescence detection.
The handbook can be obtained here or here.
Thermo Fisher Scientific