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High-throughput assay enables genome-wide protein-DNA analysis

Application note describes how Chromatrap ChIP-seq assays now enable unbiased, genome-wide understanding of protein-DNA regulatory networks.

Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). 

The new application note reports on how the ChIP-seq kit, when used with Illumina ChIP-seq library preparation kits and sequencing platforms, is able to deliver high-quality DNA for sequencing. Due to the assay’s solid-phase matrix and the option of a 96-well plate for assays, sample throughput and reproducibility is increased owing to smaller sample volumes, centrifugal wash-steps and a matrix that does not require blocking.

Compatible with direct, deep sequencing of enriched fragments, the ChIP-seq assay is shown to enable unbiased, genome-wide understanding of protein-DNA regulatory networks. In addition, the inert solid-support matrix enables reproducible capture and genome-wide amplification of landmark regulatory complexes from low amounts of input chromatin. Further the results demonstrate how the ChIP-seq can be used to deepen understanding of the genome-wide binding patterns of estrogen receptor in the human endometrium; demonstrating the utility of fast, multiplex analysis by massively parallel next-generation sequencing. To download a copy of the new application note please visit Chromatrap, a unit of Porvair Sciences

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