Automated recombinant protein synthesis/ purification system allows for rapid, small-scale in vitro synthesis and purification of 1 to 16 different proteins per run. System automation allows for a simple template-DNA-input, purified-protein-output workflow in approximately 6 hours. The E coli- based transcription/translation system is designed to maximize efficiency and yield. Purification of the expressed protein is carried out by His-tag purification using the manufacturer’s spherical nickel-magnetic nanoparticles. The net result is 1-16 samples of up to 100 ug of > 90% pure protein(s).