System automates the preparation of libraries for the Life Technologies SOLiD* Sequencer. Combining automation with solid phase reversible immobilization (SPRI) chemistry, the system facilitates the construction of consistent, quality libraries, saving researchers’ valuable time. One to ten samples can be completed within three and a half hours, and the process provides several size-selection and nick translation options. Earlier versions automated library preparation for the Illumina Genome Analyzer and the Roche GS FLX** DNA Sequencer. All three versions use a dedicated, colour-coded method card and cartridges, and library construction for all three sequencing platforms can be conducted using one instrument.