During cellular reprogramming the feeder layer can greatly affect the health of the cell culture and the success of the reprogramming. While a range of inactivated fibroblasts can be used as a support layer for pluripotent cell culture, not all will support mRNA reprogramming. Newborn Human Foreskin Fibroblasts (NuFF-RQ™) have been functionally validated to support the generation of induced pluripotent stem cell (iPSC) colonies providing an optimized feeder layer during mRNA reprogramming.
The fibroblasts have been added to the manufacturer’s range of Mouse Embryonic Fibroblasts (MEFs) and Newborn Human Foreskin Fibroblasts (NuFFs) for stem cell culture experimentation. The feeder cells are meticulously derived and comprehensively tested on mouse and human ES stem cells to ensure robust and consistent performance with every lot. Fully qualified, ready-to-use feeder cells save the time and trouble of dealing with an animal facility, dissections, cell expansion and lot-to-lot variation. Having undergone comprehensive safety tests including human pathogen and mycoplasma detection, the feeder cells significantly reduce the threat of contamination in iPSC reprogramming experiments.
More details are available at http://www.amsbio.com/Stem-Cell-Reprogramming-Qualified-Feeder-Cells.aspx