PCR reagent is designed to deliver robust high resolution melt (HRM) performance for sensitive and accurate detection of single nucleotide polymorphisms (SNP) and CpG methylation for epigenetic studies. The reagent is compatible with all HRM-capable thermal cyclers. Traditional genotyping methods, such as denaturing high-pressure liquid chromatography (dHPLC) and single-strand conformation polymorphism (SSCP), involve lengthy protocols and require multiple days for completion. Probe-based genotyping can be expensive, especially for large numbers of SNPs. HRM is a simple, rapid, and inexpensive alternative for studying genetic variation.