Normal human primary corneal epithelial cells are an alternative test method platform for toxicological assays, and can be used in research related to gene regulation, tissue development, wound healing, cell-matrix interactions, and drug development. The cells have undergone sterility testing for bacteria, yeast, fungi and Mycoplasma, as well as viral testing for HIV and HCV. A certificate of analysis is available for each lot of cells. The cells are cryopreserved in the second passage to ensure the greatest purity coupled with high viability and plating efficiency. Each lot is performance tested with a matched set of components including media, growth factors and reagents.