A quick and easy method to determine catechins in various teas after a simple solvent extraction can be found in Application Brief 150: Rapid Separation of Catechins in Tea Using Core-Shell Columns. It demonstrates use of a high-performance liquid chromatography (HPLC) core-shell column and absorbance detection to separate and detect catechins in <6 minutes. Catechins are flavonoid phytochemical compounds found primarily in green tea and—in smaller amounts—in grapes, black tea, chocolate, and wine. Catechins are considered potent antioxidants that provide protection against certain diseases, such as cardiovascular disease and cancer. Core-shell particles improve mass transfer kinetics by restricting intraparticle diffusion to the thin, porous shell while maintaining the hydraulic permeability associated with the total particle diameter. Core-shell columns can be used to increase separation efficiency without significantly increasing pressure.
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